Application of phiC31 integrase system in stem cells biology and technology: a review
نویسندگان
چکیده
منابع مشابه
Efficient reversal of phiC31 integrase recombination in mammalian cells.
Over the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. The enzyme efficiently mediates recombination between two distinct sequences, attP and attB, producing recombinant product sites, attL and attR. The reaction proceeds exclusively in a unidirectional manner, because integrase is u...
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Background: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous DNA into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene. Objectives: Identification of a novel pseudo attP site. Materials and Methods...
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ژورنال
عنوان ژورنال: Frontiers in Life Science
سال: 2018
ISSN: 2155-3769,2155-3777
DOI: 10.1080/21553769.2018.1447516